rabbit anti rat smad7  (Proteintech)

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Centella asiatica inhibits renal interstitial fibrosis by regulating Smad3 and Smad7 expression in the TGFβ signaling pathway

doi:

Figure Lengend Snippet: Primers for quantitative PCR analysis

Article Snippet: The slides were incubated with primary antibodies of the rabbit anti-rat Smad3 and Smad7 (1:100; Bioss, Beijing, China) overnight at 4°C.

Techniques: Real-time Polymerase Chain Reaction, Sequencing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Centella asiatica inhibits renal interstitial fibrosis by regulating Smad3 and Smad7 expression in the TGFβ signaling pathway

doi:

Figure Lengend Snippet: Treatment of CA inhibited Smad3 expression and increased Smad7 expression in the renal tissues of the UUO rats. CA or vehicle control was orally administered in sham- or UUO-operated rats starting on the day of the surgery for 14 days. A. Representative photomicrographs show the immuno-histochemical results of each group (400×). B. Quantification of Smad3 and Smad7 expression in each group. Error bars represent mean ± SD. *P<0.05 compared with Smad3 in sham group; #P<0.05 compared with Smad3 in UUO group; +P<0.05 compared with Smad7 in sham group; &P<0.05 compared with Smad7 in UUO group.

Article Snippet: The slides were incubated with primary antibodies of the rabbit anti-rat Smad3 and Smad7 (1:100; Bioss, Beijing, China) overnight at 4°C.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Centella asiatica inhibits renal interstitial fibrosis by regulating Smad3 and Smad7 expression in the TGFβ signaling pathway

doi:

Figure Lengend Snippet: Smad3 mRNA and protein expression in each group (mean ± SD)

Article Snippet: The slides were incubated with primary antibodies of the rabbit anti-rat Smad3 and Smad7 (1:100; Bioss, Beijing, China) overnight at 4°C.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Centella asiatica inhibits renal interstitial fibrosis by regulating Smad3 and Smad7 expression in the TGFβ signaling pathway

doi:

Figure Lengend Snippet: Treatment of CA inhibited Smad3 gene and protein expression in the renal tissues of the UUO rats. CA or vehicle control was orally administered in sham- or UUO-operated rats starting on the day of the surgery for 14 days. A. Smad3 was measured by the Western Blot; B. Quantitative data of the Smad3 mRNA and protein expression analyzed for each group. The error bars represent mean ± SD. *P<0.05 compared with Smad3 mRNA level in sham group; #P<0.05 compared with Smad3 mRNA level in UUO group; +P<0.05 compared with Smad3 protein level in sham group; &P<0.05 compared with Smad3 protein level in UUO group.

Article Snippet: The slides were incubated with primary antibodies of the rabbit anti-rat Smad3 and Smad7 (1:100; Bioss, Beijing, China) overnight at 4°C.

Techniques: Expressing, Western Blot

Journal: Materials Today Bio

Article Title: Zwitterion nanocomposite hydrogels with bioactivity and anti-adhesion properties for rapid prevention of postoperative and recurrent adhesion

doi: 10.1016/j.mtbio.2025.101811

Figure Lengend Snippet: (a, b) Representative images of immunofluorescence staining of Smad 2/3 and Smad 7 on day 7 and day 14. (c, d) Mean fluorescence intensity (MFI) of Smad2/3 and Smad7 on day 7 and day 14, respectively. (n = 3, ∗∗∗∗p < 0.0001). (e–g) Western blot analysis and quantitative of protein expressions (Smad2/3 and Smad7) on day 7, 14. (H) Molecular mechanism of pSBLA2 hydrogel in preventing postoperative abdominal adhesions. (Created with BioRender.com ).

Article Snippet: Rabbit anti-rat Smad7 antibodies were obtained from Sanying Biotechnology.

Techniques: Immunofluorescence, Staining, Fluorescence, Western Blot

Journal: Evidence-based complementary and alternative medicine : eCAM

Article Title: A Compound of Chinese Herbs Protects against Alcoholic Liver Fibrosis in Rats via the TGF- β 1/Smad Signaling Pathway.

doi: 10.1155/2019/9121347

Figure Lengend Snippet: Figure 11: Protein expression levels of hepatic TGF-𝛽1, p-Smad2, Smad2, p-Smad3, Smad3, and Smad7. Notes: A: blank control group (distilled water); B: model control group (distilled water); C: positive control group (3 g/kg⋅bw/day, positive medicine); D: low dose group (0.333 g/kg⋅bw/day, PSSS compound); E: medium dose group (0.667 g/kg⋅bw/day, PSSS compound); F: High dose group (1 g/kg⋅bw/day, PSSS compound). TGF-𝛽1, transforming growth factor-𝛽1.

Article Snippet: PVDF membranes were blocked in 5% bovine serum albumin dissolved in tris-buffered saline containing Tween20 (TBST) for 2 h and then incubated in primary rabbit antirat TGF-β1 (monoclonal, 1:1000, Abcam, USA), rabbit antirat p-Smad2, rabbit anti-rat Smad2, rabbit anti-rat p-Smad3, rabbit anti-rat Smad3 (all monoclonal, 1:1000, CST, USA), rabbit anti-rat Smad7 (polyclonal, 1:1000, Proteintech group, Wuhan, China), and rabbit anti-rat GADPH (polyclonal, 1:10000, Proteintech group, Wuhan, China) overnight at 4∘C.

Techniques: Expressing, Control, Positive Control

Journal: International Journal of Molecular Medicine

Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury

doi: 10.3892/ijmm.2018.3695

Figure Lengend Snippet: Reverse transcription-quantitative polymerase chain reaction primer sequences.

Article Snippet: According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H 2 O 2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C.

Techniques: Polymerase Chain Reaction, Sequencing

Journal: International Journal of Molecular Medicine

Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury

doi: 10.3892/ijmm.2018.3695

Figure Lengend Snippet: Target association verification between miR-20b-5p and Smad7. (A) Predicted binding site of miR-20b-5p in the Smad7 3′UTR. (B) Detection of luciferase activity by dual luciferase reporter gene assays. * P<0.05, compared with the NC group. miR-20b-5p, microRNA-20b-5p; Smad7, small mothers against decapentaplegic homolog 7; mut, mutant; wt, wild-type; NC, negative control.

Article Snippet: According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H 2 O 2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C.

Techniques: Binding Assay, Luciferase, Activity Assay, Mutagenesis, Negative Control

Journal: International Journal of Molecular Medicine

Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury

doi: 10.3892/ijmm.2018.3695

Figure Lengend Snippet: Survival rates of rats.

Article Snippet: According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H 2 O 2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C.

Techniques:

Journal: International Journal of Molecular Medicine

Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury

doi: 10.3892/ijmm.2018.3695

Figure Lengend Snippet: Results of LVEDV, LVEDD, LVESV and LVESD by echocardiography (n=5).

Article Snippet: According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H 2 O 2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C.

Techniques:

Journal: International Journal of Molecular Medicine

Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury

doi: 10.3892/ijmm.2018.3695

Figure Lengend Snippet: Echocardiograph results of LVEEF and LVEFS (n=5).

Article Snippet: According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H 2 O 2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C.

Techniques:

Journal: International Journal of Molecular Medicine

Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury

doi: 10.3892/ijmm.2018.3695

Figure Lengend Snippet: Echocardiograph results of LVESP and LVEDP (n=5).

Article Snippet: According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H 2 O 2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C.

Techniques:

Journal: International Journal of Molecular Medicine

Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury

doi: 10.3892/ijmm.2018.3695

Figure Lengend Snippet: Myocardial infarct size in rats in each group. (A) Triphenyltetrazolium chloride staining results of rats in each group. (B) Comparison of myocardial infarct size in rats in each group. * P<0.05, compared with the sham group; # P<0.05, compared with the IR group; & P<0.05, compared with the miR-20b-5p inhibitors + si-Smad7 group. miR-20b-5p, microRNA-20b-5p; Smad7, small mothers against decapentaplegic homolog 7; si-, small interfering RNA; NC, negative control; IR, ischemia-reperfusion; INFS, infarct size.

Article Snippet: According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H 2 O 2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C.

Techniques: Staining, Small Interfering RNA, Negative Control

Journal: International Journal of Molecular Medicine

Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury

doi: 10.3892/ijmm.2018.3695

Figure Lengend Snippet: Ultrastructure of cardiomyocytes in rats in each group (n=3). Images at magnification, ×3,000. miR-20b-5p, microRNA-20b-5p; Smad7, small mothers against decapentaplegic homolog 7; si-, small interfering RNA; NC, negative control; IR, ischemia-reperfusion.

Article Snippet: According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H 2 O 2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C.

Techniques: Small Interfering RNA, Negative Control

Journal: International Journal of Molecular Medicine

Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury

doi: 10.3892/ijmm.2018.3695

Figure Lengend Snippet: Hematoxylin and eosin staining of the myocardium in rats in each group (n=3). Images at magnification, ×200. miR-20b-5p, microRNA-20b-5p; Smad7, small mothers against decapentaplegic homolog 7; si-, small interfering RNA; NC, negative control; IR, ischemia-reperfusion.

Article Snippet: According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H 2 O 2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C.

Techniques: Staining, Small Interfering RNA, Negative Control

Journal: International Journal of Molecular Medicine

Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury

doi: 10.3892/ijmm.2018.3695

Figure Lengend Snippet: Masson's staining of myocardial tissue from rats in each group. (A) Masson's staining of myocardial tissue from rats in each group (magnification, ×400). (B) Changes in CVF and PVCA in rats in each group are shown in a bar graph. * P<0.05, compared with the sham group; # P<0.05, compared with the IR group; & P<0.05, compared with the miR-20b-5p inhibitors + si-Smad7 group. CVF, myocardial collagen volume fraction; PVCA, perivascular collagen area; miR-20b-5p, microRNA-20b-5p; Smad7, small mothers against decapentaplegic homolog 7; si-, small interfering RNA; NC, negative control; IR, ischemia-reperfusion.

Article Snippet: According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H 2 O 2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C.

Techniques: Staining, Small Interfering RNA, Negative Control

Journal: International Journal of Molecular Medicine

Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury

doi: 10.3892/ijmm.2018.3695

Figure Lengend Snippet: Immunohistochemical results of rats in each group (n=3). (A) Immunohistochemical results (magnification, ×400). (B) Comparison of the Smad7-positive rate among all rat groups. miR-20b-5p, microRNA-20b-5p; Smad7, small mothers against decapentaplegic homolog 7; si-, small interfering RNA; NC, negative control; IR; ischemia-reperfusion; * P<0.05, compared with the sham group; # P<0.05, compared with the IR group; & P<0.05, compared with the miR-20b-5p inhibitors + si-Smad7 group.

Article Snippet: According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H 2 O 2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C.

Techniques: Immunohistochemical staining, Small Interfering RNA, Negative Control

Journal: International Journal of Molecular Medicine

Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury

doi: 10.3892/ijmm.2018.3695

Figure Lengend Snippet: Expression of miR-20b-5p and mRNA expression of TGF-β1/Smad signaling pathway-related proteins in myocardial tissue from rats in each group. * P<0.05, compared with the sham group; # P<0.05, compared with the IR group; & P<0.05, compared with the miR-20b-5p inhibitors + si-Smad7 group. miR-20b-5p, microRNA-20b-5p; Smad3, small mothers against decapentaplegic homolog 3; Smad7, small mothers against decapentaplegic homolog 7; si-, small interfering RNA; TGF-β1, transforming growth factor-β1; NC, negative control; IR, ischemia-reperfusion.

Article Snippet: According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H 2 O 2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C.

Techniques: Expressing, Small Interfering RNA, Negative Control

Journal: International Journal of Molecular Medicine

Article Title: MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-β/Smad signaling pathway in a rat model of ischemia-reperfusion injury

doi: 10.3892/ijmm.2018.3695

Figure Lengend Snippet: Expression of TGF-β1/Smad signaling pathway components in myocardial tissue from rats in each group. (A) Banding diagram of the expression of TGF-β1/Smad signaling pathway components in myocardial tissue from rats in each group, (B) Histogram of the expression of TGF-β1/Smad signaling pathway components in myocardial tissue from rats in each group. * P<0.05, compared with the sham group; # P<0.05, compared with the IR group; & P<0.05, compared with the miR-20b-5p inhibitors + si-Smad7 group. miR-20b-5p, microRNA-20b-5p; Smad3, small mothers against decapentaplegic homolog 3; Smad7, small mothers against decapentaplegic homolog 7; si-, small interfering RNA; TGF-β1, transforming growth factor-β1; NC, negative control; IR, ischemia-reperfusion.

Article Snippet: According to the conventional method of immunohistochemical staining, following the elimination of endogenous peroxidase activity in 0.3% H 2 O 2 − methanol solution at room temperature for 10 min, a 1:100 dilution of rabbit anti-rat Smad7 antibody (cat. no. BA1399; 1:100; Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) was added to the cardiac tissue for 16 h at 4°C.

Techniques: Expressing, Small Interfering RNA, Negative Control